Performance Comparison of Gel Imaging Devices
نویسندگان
چکیده
This paper presents a method for gel imaging device comparison. Fully amplified DNA product was diluted to have six different concentrations. They were loaded into separate wells of a gel and electrophoresed. The images of the prepared gel were captured with the chosen cameras by installing them in turn on the mount of a gel documentation system. Two industrial video cameras, a compact digital camera, and a DSLR digital camera were selected for the comparison. Visual inspection of the images showed that the gel images from the industrial video cameras did not present the bands contrast to the digital cameras. Both digital cameras having similarly superior performance than the industrial ones were compared quantitatively using software packages for gel image analysis. The linearity between the band intensity and DNA density was compared for them, and results showed that the two cameras have similar performance.
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